The present technical study presented the mechanical dissociation protocol of adipose tissue from manual liposuction, as well as showed the immunobiological characterization (immunophenotyping), cell viability of the stromal vascular fraction of human adipose tissue, and cell concentration per milliliter.

Abstract: The present technical study presented the mechanical dissociation protocol of adipose tissue from manual liposuction, as well as showed the immunobiological characterization (immunophenotyping), cell viability of the stromal vascular fraction of human adipose tissue, and cell concentration per milliliter. It was observed that the technique of the present study obtained a characterization yield of the stromal vascular fraction of the adipose tissue in terms of cell viability, cell concentration per milliliter, and immunophenotyping comparable and superior to most published studies with good scientific evidence.

Conclusion: It was concluded that the technique of the present study obtained a characterization yield of the stromal vascular fraction of adipose tissue in terms of cell viability, cell concentration per milliliter, and immunophenotyping comparable and superior to most published studies with good scientific evidence.

https://mednext.zotarellifilhoscientificworks.com/index.php/mednext/article/view/241